Summary of Morus alba
Primary Information, Benefits, Effects, and Important Facts
Morus Alba is the White Mulberry, although the fruits are what 'White Mulberry' refers to the stems and leaves are also commonly used as a tea and, more recently, in supplements as ethanolic/ethyl acetate extractions (supplements, or a wine extraction) appear to concentrate the bioactives. The term Morus can be seen as synonymus with the common word 'Mulberry' where the species of Alba literally means White (derived from the Latin term Albus). Other Morus herbs differ in their coloration, such as Morus Nigra (Black Mulberry) and are not the subject of this article.
White Mulberry has had all parts of it used in Traditional Chinese Medicine for a variety of purposes, but currently most evidence on Morus Alba is in regards to its anti-diabetic properties. Surprisingly, there is a large body of repeated rodent evidence to suggest it effective in reducing blood sugar regardless of previous state (toxin-induced diabetic, diet-induced diabetic, genetically diabetic or normal rodents) but with no current human evidence.
It merely appears to inhibit absorption of carbohydrates from the intestines, with most potency on inhibiting sugar absorption (fairly weak in inhibiting starch absorption, but is synergistic with Hibiscus Sabdariffa on this which would make a nice combination). For the most part, this is tied back into the iminosugar compound known as 1-deoxynojirimycin which is a glucose molecule with a nitrogen attached it it; it inhibits enzymes that have affinity for sugars via competitive inhibition where the enzyme is attracted to the glucose structure but cannot act effectively due to the nitrogen group (which does not exist on sugars normally and hinders the enzyme's functions)
There appear to be promising cognitive effects associated with Morus Alba as well, with some evidence suggesting it can increase memory formation and cognition to a level similar to Piracetam; interesting, there are some pyrrole alkaloids in Morus Alba (a structural class of molecules that Piracetam belongs to) but these have not yet been connected to the observed cognitive benefits.
Morus Alba may also have respectible benefits to cardiovascular health (with improvements in circulating lipids and cholesterol, with fairly potent reductions in artherosclerotic plaque buildup possibly related to potent in vitro anti-inflammatory properties) but similar to the other claims these have not yet been tested in humans.
Currently, the evidence suggests that Morus Alba is a highly promising functional food and tea product that may have benefit as a supplement especially in regards to cognition and glucose control but currently does not have sufficient evidence to suggest how potent these benefits are in humans and whether or not Morus Alba is a 'go-to' supplement.
Things To Know & Note
Also Known As
White Mulberry, Karayamaguwa, Sohakuhi, Sang-Bai-Pi, Ramulus Mori
Goes Well With
Hibiscus Sabdariffa (Roselle) as it augments the efficacy in inhibiting the α-amylase enzyme which digests starch, the carbohydrate digestive enzyme which Morus Alba is weakest in inhibiting
Caution NoticeExamine.com Medical Disclaimer
The fruits of Morus Alba (White Mulberries) have different properties than teas made from the stems or leaves; the latter is more anti-diabetic while cognitive aspects may be in the latter but have only currently been demonstrated with the former
How to Take Morus alba
Recommended dosage, active amounts, other details
For the purpose of reducing carbohydrate absorption and glucose spikes following a meal, morus alba must be consumed alongisde said carbohydrate source. The dosage appears to be 500-1,000mg/kg in rat studies (assuming about a 0.11% 1-deoxynojirimicin content) which is an estimated human dose of:
5,400-11,000mg for a 150lb person
7,300-14,500mg for a 200lb person
9,000-18,000mg for a 250lb person
Concentrated extracts may reduce the above requirement, so a 10:1 concentrated extract (for the 1-deoxynojirimicin content) would then require 900-1,800mg at the heaviest weight.
For inflammation and other health related issues (such as uric acid), the rat dose appears to be in the range of 20-200mg/kg which is an estimated human dose of:
220-2,200mg for a 150lb person
300-2,900mg for a 200lb person
400-3,600mg for a 250lb person
Human Effect Matrix
The Human Effect Matrix looks at human studies (it excludes animal and in vitro studies) to tell you what effects morus alba has on your body, and how strong these effects are.
|Grade||Level of Evidence [show legend]|
|Robust research conducted with repeated double-blind clinical trials|
|Multiple studies where at least two are double-blind and placebo controlled|
|Single double-blind study or multiple cohort studies|
|Uncontrolled or observational studies only|
Level of Evidence
? The amount of high quality evidence. The more evidence, the more we can trust the results.
Magnitude of effect
? The direction and size of the supplement's impact on each outcome. Some supplements can have an increasing effect, others have a decreasing effect, and others have no effect.
Consistency of research results
? Scientific research does not always agree. HIGH or VERY HIGH means that most of the scientific research agrees.
|Minor||- See study|
Scientific Research on Morus alba
Click on any below to expand the corresponding section. Click on to collapse it.
Morus Alba (of the family Moraceae), sometimes referred to as Mori Albae or White Mulberry, is a medicinal fruit (with roots and bark also having some medicial usage) with Traditional Chinese Medicine using White Mulberry for the purposes of antiphlogistic, diuretic, antitussive, expectorant, antiheadache, and antipyretic. Other names given to Morus Alba include Karayamaguwa (Japanese) when referring to the plant in general and Sohakuhi if referring to the medicinal root bark; Chinese terminology for the root bark is Sang-Bai-Pi and in english it is referred to as Ramulus Mori (branch of Mori).
When tea is made from Mulberry, it is referred to as a Sang-Yup tea when made from the leaves and a Sang-Ji tea when made from the stems.
White Mulberry (Morus Alba) is a Traditional Chinese Medicine where, although all parts have been used, the stems seem to be most focused on; teas have been made from the stems
The term 'Mulberry' refers to the genera of Morus, with 'White Mulberry' specifically referring to the species of Alba in the Morus genera (Morus Alba). Although the term Mulberry is sometimes used to refer to Morus Alba in particular due to its popularity, it may also refer to other species such as Morus nigra (Black) or Morus Rubra (Red). There are approximately 10-16 species of Mulberry in cultivation in subtropical, warm and temperate regions of Asia, Africa and North America and up to 24 in total with one varient known as Morus laevigata (Shahtoot) being used as a common hybrid species in Pakistan.
Mulberry is a general term for the genera of Morus, White Mulberry being specific for Morus Alba
The fruit itself tends to contain 81.72+/-2.25% Moisture, 0.57+/-0.11% Ash (minerals and other noncombustible compounds) 0.48+/-0.11% Lipid, 1.55+/-0.30% Protein, and 1.47+/-0.15% Fiber; giving the total carbohydrate content of the fresh fruit 14.21+/-1.01% (76% dry weight) and the fresh fruit having 67kcal per 100g. This is similar to both Morus Nigra and Morus laevigata except that Morus Nigra has a much higher fiber content (11% wet weight).
A variety of non-caloric bioactives that, for the most part, are unique to Morus Alba include:
Albanol F and G, which differ from the other 'Albanol' molecules as they also possess alternate names; Albanol F can also be referred to as either Kuwanone G or Moracenin B, while Albanol G can be referred to as Kuwanone F or Moracenin A
Cyclomulberrin and cyclocommunol
Pyrrole Alkaloids such as Morrole A
Loliolide (high concentrations in the hot water extraction of the leaves)
3′-Geranyl-3-prenyl-2′,4′,5,7-tetrahydroxyflavone and 3′,8-Diprenyl-4′,5,7-trihydroxyflavone
In regards to the above molecules that are relatively unique to Morus Alba (they tend to be named after Morus or Alba) many of them have not yet been shown to be the causative active ingredients. It is definitely possible a unique bioactive here underlies some benefits of Morus Alba, but for the most part the main bioactives of concern are in the next sections
With various flavonoid molecules (that may be common to many plants) being:
Resveratrol and Oxyresveratrol (0.2358mg/g and 4.8398mg/g in 95% ethanolic extract of branches) and the diglucoside of oxyresveratrol, Mulberroside A at 0.8-1.5%. Mulberrosides B and cisMulberroside A have also been reported with two glycosides of oxyresveratrol (4'-O-β-Glucopyranoside and 3-O-β-Glucopyranoside) and a diglycoside of resveratrol known as resveratrol 3,4'-di-O-β-D-glucopyranoside.
Luteolin (2.1mcg/g leaf, bark and branch, 4.3mcg/g root and none in fruits), Apigenin (3mcg/g roots, 4.2mcg/g bark, 43.8mcg/g stem, 3.5mcg/g fruits, and 42.7mcg/g leaf), and 6-geronylapigenin
Phenophorbide A methyl ester 132(S)-hydroxypheophorbide A methyl ester; breakdown products of chlorophyll found in the leaves
4-hydroxycinnamic acid (1.0839mg/g in 95% ethanolic branch extract), 7-hydroxycoumarin (Umbelliferon, 0.2382mg/g in 95% ethanolic branch extract) caffeic acid (7.3mcg/g leaf and 17.2mcg/g fruit; none in root, bark, or stem) and dicaffeic acid
Iminosugars such as 1-deoxynojirimicin (a pipiridine alkaloid) at 2.24-3.08mg/g (stems), 0.62-1.61mg/g (young/fresh leaves), or 0.47-0.96mg/g (older leaves) and D-fagomine (25-103mg/kg in the leaves with 185mg/kg also being reported; both in the reported range of 50-660mg/kg)
Polyprenyl compounds (linear hydrophobic isoprenoid alcohols)
Rutin (Quercetin-3-O-Rutinoside) at 0.0766mg/g in a 95% ethanolic branch extract and most content being centralized to leaves and fruits (179.1-293.5mcg/g); similar levels of free Quercetin, Hyperin, and Isoquercetin, with up to two-fold (0.1270-0.1402mg/g) of Kaempferol and Butein
Chlorogenic Acid (19.1mcg/g stem, 28.6mcg/g bark, 47.3mcg/g branch, 226.9mcg/g fruit, 92.2mcg/g leaf) and Umbelliferone (15.7mcg/g in stem, 289.6-538.7mcg/g in bark and root; none in leaves or fruit)
Taxifolin (6.53-21.42mcg/g) and Taxifolin Hexoside (undetectable to 9.06mcg/g)
Volatiles (aromatic and flavorants) at 0.36+/-0.03% (leaves) and 0.25+/-0.03% (stems); of which major ones include n-hexanoic acid (19.83mcg/g stems) and (E)-β-ionone (9.32mcg/g), dihydroctinidiolide (9.65mcg/g) and 3-methylbutyl hexadecanoate (7.7mcg/g) in the leaves.
The Quercetin class of molecules, with notice to the 3-(6-malonylglucoside) conjugate, appear to play a role as do the stilbene class of molecules (resveratrol and oxyresveratrol, where the glycosides known as 'Mulberrosides' are unique to this plant). Beyond that, the iminosugars appear to play a large role in anti-diabetic effects. Ursolic acid and chlorogenic acid cannot be ruled out for anti-diabetic effects, while the anthocyanins probably can be ruled out since they are blue-red pigmentations and their content is low enough for this to still be called 'white mulberry' (they are at higher levels in Morus Nigra; Black Mulberry)
With the caloric bioactives being:
A variety of larger molecules with the designation of Kuwanone or Albanol appear to be, structurally, a combination of an isoprenylated flavonoid with a chalcone structure; both of the latter also occurring in Morus Alba. These meta structures are referred to as Diels-Alder Adduct formation products, named after the specific chemical reaction that occurs in the plant to create them.
Total Phenolics are highest in the leaf and lowest in the stem (fruit intermediate) where flavonoids are lowest in the fruit, with the leaves still the highest concentration. Comparatively, both Morus Alba and Morus Nigra seem to have similar levels of phenolics, with some studies noting a higher level in Nigra and others in Alba.
In general, the leaves of Morus Alba tend to have fairly potent anti-oxidant effects (IC50 of 20.10+/-0.78mcg/mL in the DPPH assay ex vivo) which extends to teas. Ethanolic extracts (of any part of Morus Alba) are more potent than aqueous hot water extracts, however, with acetone extracts slightly better than ethanolic.
Phenolics (and the subset of phenolics known as 'flavonoids') are high in all parts of Morus Alba, with higher content in the stems and leaves; although they can be leeched into teas (hot water extractions) their levels are concentrated to higher levels in ethanolic and acetone extractions
Mulberroside A (oxyresveratrol diglucoside) appears to have a less than 1% oral bioavailability in the rat due to extensive metabolism to oxyresveratrol; oxyresveratrol appears to have comparatively better absorption in Caco-2 cells, and is subject to P-glycoprotein efflux proteins.
Limited evidence all around, but the glycoside of oxyresveratrol appears to need to be metabolized into the aglycone oxyresveratrol before most absorption takes place
Most components of Mulberry, if they don't have their own respective page (like Quercetin or Chlorogenic Acid do) lack pharmacokinetic data
Mulberroside A can be metabolized to either Oxyresveratrol or one of two conjugates of oxyresveratrol (Oxyresveratrol-2-O-β-d-glucuronosyl via glucuronidation and oxyresveratrol sulfate), as detected in rat feces following oral administration; these fecal doses of conjugates account for up to half of the ingested oral dose of Mulberroside A.
Mulberroside A is surprisingly stable under varying pH conditions (surviving gastric juices for up to an hour ex vivo) and appears to be metabolized when incubated with colonic microflora.
Moracin M is a PDE4 inhibitor, inhibiting the D2 and B4 subsets with IC50 values of 2.9μM and 4.5μM respectively with weak inhibitory potential on PDE5 (13-fold less inhibition than PDE4s) and PDE9 (30-fold less). Moracin C also inhibited PDE4D2 with lesser (26μM) potency, underperformed to the active control (roflumilast at 0.46nM) and compared to luteolin (a nonspecific PDE inhibitor from artichoke extract), Moracin M outperforms Luteolin's IC50 of 19.1uM and the inhibition by resveratrol (14μM). PDE4 is a camp-specific phosphodiesterase enzyme, and its inhibition is thought to enhance synaptic function via elevating hippocampal cAMP concentrations (evidence by studies on Rolipram, a potent PDE4 seletive inhibitor).
Moracin M is a PDE4 inhibitor, more potent than other molecules derived from nutraceuticals (but still not as effective as the prototypical reference drug of Rolipram). Although PDE4 inhibition has been shown to enhance cognition (studies using Rolipram), and Morus Alba has been shown to increase cognition this has not yet shown to be due to PDE4 inhibition
An ethyl acetate fraction of Morus Alba appears to positively modulate GABA(A) receptors, which is thought to be due to the sanggenon compounds; with concentrations between 1-100µM exerting EC50 values in the 13-17µM range and maximal potency around 30µM or more (curve of dose-response being attenuated at higher concentrations) being in the range of 718-750% increased activation of GABA(A) receptors and 30µM activating the receptors inherently, although much less potent than GABA itself. This study noted the potency of positive modulation was similar to 8-lavandulyl flavanoids from Sophora Flavescens and Wogonin from Scutellaria baicalensis. Anti-dopaminergic effects of the ethyl acetate fraction (unknown bioactive) have also been shown to augment phenobartitol induced sleep.
Two independent mechanisms suggest possible relaxation, anxiolytic, or sedative properties of unknown potency
Oral doses of 50, 100, or 200mg/kg of an extract from the fruits of Morus Alba over a period of a week can dose-dependently induce Nerve Growth Factor (NGF) release in the rat hippocampus, with downstream molecular targets (neurite outgrowth, synaptic formation, and cAMP induction) as well as enhanced learning being noted. Another study assessing the anti-stress effects and using an ethyl acetate fraction noted that 25-100mg/kg over 21 days was actually associated with a trend to outperform non-stressed control on step-down latency, indicative of memory improvements (no unstressed group given Morus Alba).
Appears to be able to enhance memory formation and cognition in otherwise normal rats. Limited studies and no bioactive currently found to be causative, but these occur with reasonable oral doses (50-100mg/kg in rats correlates to 8-16mg/kg in humans; this may simply be a few fruits)
A mulberry extract with a high Cyanidin-3-Glucoside content, at 1-50mcg/mL was able to reduce the 95.1+/-1.6% cell death induced by oxygen/glucose deprivation to 37.6+/-5.3% at the highest concentration and to 61.4-61.5% viability at lower concentrations of 5-10mcg/mL, and was seemingly ineffective against glutamate-induced neurotoxicity.
The methanolic extract of Morus Alba may possess anti-dopaminergic effects, as it has been shown to augment haloperidol and metoclopramide induced catalepsy in mice when Morus Alba is ingested at 50-200mg/kg and has also prolonged phenobartitol induced sleep time while reducing amphetamine-induced fights in mice. Alkaloids and saponins were determined to be in this extract, and an attenuation of the dopamine-induced vas deferens contractions was also noted.
Excessive usage of haloperidol (dopamine D2 antagonist) is associated with excitotoxicity, which Morus Alba appears to attenuate in vivo.
Possible anti-dopaminergic effects which may be tied into neuroprotection; practical relevance unknown
In a chronic restraint stress model, the ethyl acetate fraction of Morus Alba (25-100mg/kg) given 60 minutes prior to the stress test (done daily for 10 days) was able to prevent the cognitive deficits induced by stress, with the active control of 1mg/kg Diazepam outperforming all doses but 100mg/kg on day 1 (equal potency to 100mg/kg) and over 10 days all doses of Morus Alba becoming more effective than 1mg/kg Diazepam (passive shock avoidance and elevated plus maze tests). This study noted preservation of oxidant enzyme changes (altered with stress, somewhat normalized with both Morus Alba and Diazepam) and the serum corticosterone levels were normalized in all treated stress groups. Another study using chronic stress testing (21 days) with the same dose of the same extraction was able to preserve memory losses associated with chronic stress (Diazepam inactive) with the two lower doses (25-50mg/kg) trending to outperform non-stressed control. Both studies note normalization of serum glucose and organ weight alterations, biomarkers of stress.
Possible adaptogenic and anxiety reducing effects of similar potency to 1mg/kg Diazepam, repeated studies but all by the same research group
Morus Alba appears to inhibit pancreatic lipase with an IC50 of 3.41+/-0.67mg/L, although none appear to be present in the leaves. Another study noted an IC50 of 244.94+/-83.96ug/mL, which was effectively the weakest herb tested.
Technically can inhibit fatty acid absorption, is comparatively very weak at doing so
Intravenous administration of the methanolic extract of the root bark leads to dose-dependent reductions in blood pressure in rabbits, which is due to the bioactives kuwanon G and H (0.2% and 0.13% of the methanolic extract). These results have been replicated in other studies, where Kuwanon G and H were temporarily designated alternate names (Albanins or Moracenins).
When the water extract is given to mice fed an atherogenic diet, an attenuation of the development of high blood pressure occurs with 100-250mg/kg over 14 weeks; this study noted that both doses outperformed the active control fluvastatin and reduced blood pressure to lower than the control group.
May preserve blood pressure in disease states (diabetes or artherosclerosis) where blood pressure is increased
A study in HUVEC cells (endothelial) with the leaves of Morus Alba at 400mcg/mL (deemed a physiologically relevant dose citing this study) is able to inhibit the expression of P‐selectin and fractalkine induced by resistin, with similar potency to 20uM Curcumin (both fully abolishing the effects of resistin) thought to be related to attenuating NADPH oxidase activity (abolished with Morus Alba, reduced to 30% of control with Curcumin). Resistin induces P-Selection and fractalkine expression in endothelial cells, which increases monocyte adhesion and may be pro-artherosclerotic and in HUVEC cells Morus Alba was shown to inhibit monocyte binding to a potency correlating greatly with NADPH oxidase activity.
In LDLR-/- mice prone to artherosclerotic lesions fed 3% of the diet as Mulberry leaf powder for 8 weeks, artherosclerotic lesions were greatly reduced and partly attributed to the Quercetin content of the leaves (with 0.5% Quercetin also reducing lesions, and 0.5% Quercetin-3,6-malonylglucoside being intermediate in potency to the high dose Mulberry and equal dose Quercetin). As the mulberry group had 0.4% Quercetin content overall yet outperformed the other groups, it appears other bioactives are at play. The entire leaf extract itself appears to reduce the artherogenic index to the same level as Fluvastatin (3mg/kg), thought to be related to mixed anti-oxidant and anti-inflammatory effects but with a concomitant increase in HDL-C.
Appears to protect against artheroscelrosis, with the bioactive of Quercetin-3,6-Malonylglucose playing a major role (more potent than the Quercetin aglycone). Relative to other plants, this is a respectable potency in animal models but lacks human studies
In vitro, Morusinol can inhibit clotting with IC50 values of 13.4+/-5.4ug/mL for Collagen-induced clotting 19.8+/-3.5ug/mL for Arachidonic Acid-induced clotting with no significant effect on thrombin-induced clotting. This was thought to be due to inhibition of thromboxane 2 (TBX2) production, which reached 99% inhibition at 30mcg/mL (a potency requiring 200mcg/mL Aspirin).
Morusinol, a prenylated flavonoid given at 20mg/kg bodyweight, occlusion time was prolonged 90% over control. Compared to the active control of Aspirin at 20mg/kg, Morusinol significantly outperformed the ability of 30mg/kg Aspirin to increase occlusion time by 30%.
Limited evidence with little practical application thereof, but Morusinol seems more potent than an equal dose of Aspirin at inhibiting blood clotting
Mechanistically, a study noting lipase inhibition (preventing absorption of fatty acids) noted that Morus Alba fruits had an IC50 against pancreatic lipase of 244.94+/-83.96ug/mL (weakest tested plant) and a slight inhibition of cholesterol uptake was noted in Caco-2 cells (76.10+/-3.98 of control) but was much less than black pepper and green tea catechins and no compound outperformed Ezetimbe. A weak inhibition of HMG-CoA activity was noted (64.57+/-5.97 inhibition at 100ug/mL) which underperformed relative to 0.4mcg/mL Pravastatin (103.96+/-4.08) and 100mcg/mL Horseradish tree (106.46+/-3.70).
Morus Alba can activate both α1 and α2 isoforms of AMPK in skeletal muscle (a possible anti-diabetic and anti-lipidemic mechanism), which noted rapid activation to 1.6-fold higher than baseline after incubation with 4.28mg/mL Morus Alba and up to 1.9-fold at 14.3mg/mL.
Seems to have weak effects on preventing lipid and cholesterol uptake and weak effects in attenuating cholesterol synthesis; may reduce lipids via AMPK activation
A study in LDLR-/- mice noted that total cholesterol was reduced (and LDL trended to be reduced) with 3% Mulberry leaf extract or 0.5% of the diet as Quercetin-3,6-Malonylglucoside, but not with 0.5% Quercetin, over 8 weeks of supplementation.
In otherwise normal rats given an artherogenic diet, the inclusion of 100-250mg/kg Morus Alba water extract can slightly attenuate the increases in total cholesterol, LDL-C, and Triglycerides while promoting HDL-C levels to above that of control and artherogenic control. This increase in HDL-C was the reason Morus Alba rivalled the active control Fluvastatin on the artherogenic index, since Fluvastatin had greater reductions in the other three parameters. Other studies using high-fat fed rats (which then get hyperlipidemia) note reductions in serum triglycerides and LDL-C, with no seeming effect on rats with normal lipid profiles.
Decent efficacy in reducing blood lipids, although the increase in HDL-C ('good' cholesterol) appears to be larger than other herbs despite the reductions seen in LDL-C and Total Cholesterol being less potent
In assessing compounds that may have anti-obese effects, 20uM of various prenylated flavonoids (kuwanon A, C and T; Morusin and sanggenon F) and two triterpenoids (Uvaol and betulinic acid) showed inhibitory effects on triglyceride accumulation into preadipocytes (3T3-L1) with potency greater than 34%, which was the active control of Quercetin at 20uM, with the most potent being Sanggenon F at 56.0% inhibition at 20uM. A mixture of Morus Alba with two other herbs (Lemon Balm and Artemisia Capillaris) appears to inhibit adipogenesis in preadipocytes, associated with a downregulation of PPARγ and aP2 mRNA by 34% and 37%. In rats given the leaf extract (250-100mg/kg) for 9 weeks, it is noted that the expression of Cyp4a2 and Cyp4a3 (genes encoding the rate limit of omega-oxidation of long chain fatty acids), Phyh (encodes the rate limit for alpha-oxidation of 3-methyl branched fatty acids) and the expression of CPT1A (rate limiting step of beta-oxidation) were both upregulated; some products associated with short-chain fatty acids were downregulated (Hsd17b10, Acaa2, and Echs1). In vivo, the enhancement of oxidative metabolism was met with a decrease in oxidative stress due to antioxidant properties of Mulberry.
Morus Alba in general can increase glucose uptake into fat cells in a concentration-dependent manner, with 5-45mcg/mL increasing glucose uptake (via GLUT4 translocation) by 31-54%, which are mediated via PI3K activation (possibly insulin-dependent) and may be due to gallic acid.
Appears to beneficially influence gene expression in the liver and may increase glucose uptake into fat cells (without enhancing their proliferation, which normally occurs after glucose uptake)
Melanin-concentrating hormone (MCH) mostly in the lateral hypothalamus and zona incerta (of the CNS) appears to act on its receptor subtype 1 (MCH1) to activate hunger, and antagonism of this receptor is seen as a therapeutic target to reduce food intake.
The ethanolic extract of Morus Alba has been shown to inhibit MCH-induced calcium influx into cells in a concentration dependent manner (10-100mcg/mL) and oral ingestion of this extract (100-500mg/kg in two divided doses) over 2 weeks (much weaker than sulbutiamine as active control) and prolonged over 22-32 weeks (whereas the benefits of sulbutiamine attenuated to the same degree as Morus Alba, which was constant). The overall weight loss in this study attributed to reduced food intake was 3.1% (250mg/kg) and 4.9% (500mg/kg).
Another study in rats measuring food intake replicated a suppression of food intake when the 50% ethanolic leaf extract was used at either 3% or 6% of the diet, but it was not to a degree where body weight after 60 days was significantly different (despite being lower in the first few weeks).
Limited evidence, but there appears to be a bioactive in Morus Alba that acts as an MCH antagonist and reduces food intake. This has been noted to occur with high dose ethanolic extracts of Morus Alba in studies intentionally looking for how it affects food intake and by studies who merely noted it as a side-effect of treatment
One study using 3% or 6% ethanolic leaf extract over 60 days noted that treated rats had a reduction in perirenal, but not epididymal, adipose tissue without any noted dose-dependence. 250, 500, or 1000mg/kg Mulberry leaf powder for 7 weeks in normal rats given a high fat diet failed to significantly modify body weight despite decreasing serum levels of NEFAs and Triglycerides.
One study using genetically obese mice given a mixture of Morus Alba, Artemisia capillaris, and Melissa officinalis (found to have anti-angiogenic properties by the same researchers) noted reduced rate of weight gain associated with the mixture of herbs over 5 weeks and thought to be due to inhibiting angiogenesis; dosage of active ingredients not disclosed.
Some beneficial trends noted in body fat disposition, but they are unreliable and limited; there are numerous studies noting no significant influence on body weight when food intake is not affected. It is possible weight loss only occurs with Morus Alba secondary to reduced food intake
Morus Alba leaves appear to inhibit carbohydrate digestive enzymes with an IC50 value of 0.59+/-0.06mg/mL (maltase) and 0.94+/-0.11mg/mL (sucrase) with this study noting the IC50 of Morus Alba leaf extract on α-amylase being more than 5mg/mL but other studies with a confirmed (0.11%) 1-deoxynojirimycin content noting an IC50 of 324.5 μg/mL on amylase (and noted a similarly potent IC50 on alpha-glucoside enzymes at 41.0 μg/mL) and 1440μg/mL with an isopropanolic extract. This inhibition has been noted with hot water extracts (teas made from the leaves) with good inhibition at a 3-5 minute steep time.
The former study notes that Morus Alba outperformed other herbs such as Clitoria Ternatea (minimal efficacy on all enzymes) and chrysanthemum, with both Mulberry and Chrysanthemum being synergistic with Roselle extract (Hibiscus sabdariffa). Inhibition of carbohydrate digestive enzymes have been replicated on sucrase and maltase, and extends to palatinase; a lack of efficacy has been noted on trehalase and lactase, and competition on disaccharidase enzymes appears to be competitive.
When looking at bioactives that may underlie the effects above, it is thought that isoquercitrin and astragalin may play roles on the α-glucosidase enzymes. D-Fagomine (found in water extracts of the leaves) is also known to be able to attenuate the rate of glucose absorption due to its structural similarity. This also applies to the structurally related iminosugar 1-deoxynojirimycin, which is an α-glucoside inhibitor of comparable potency (IC50 values) to Arcabose (with 1-deoxynojirimycin also inhibiting α-amylase, but much weaker than Arcabose).
May reduce or slow some carbohydrate absorption from disaccharides (sucrose and maltose) and shows some synergism with Hibiscus sabdariffa in doing so. A few ingredients may be causative here, but most of the inhibition appears to be related to 1-deoxynojirimycin (an iminosugar)
Relative to other herbal options, Morus Alba (particularly teas made from the stems and leaves, as they have a higher 1-deoxynojirimycin content than the fruits) seems to be a respectable option for reducing the absorption of carbohydrates (although its effects on starches and lactose are subpar relative to its efficacy in inhibiting other disaccharides)
A hot water extraction of Morus Alba leaves (high Loliolide content) appears to enhance glucose uptake into isolated adipocytes (fat cells) via insulin dependent and independent means. These effects have been noted in vitro with the 20kDa glycoprotein Morin 20K.
Quercetin-3,6-Malonylglucoside (Q3MG) appears to enhance the activity of glucokinase (2.81-fold) and ATP Citrate Lyase (2.27-fold) after oral ingestion at 0.7mg/kg in rats fed a high fat diet, relative to high fat control, over 8 weeks. This study noted, however, that PGC-1a gene activity was reduced 2.19-fold, Heat shock protein 1B down 9.53-fold, and that Cdkn1A activity was reduced 10.19 fold (high magnitude changes that should be noted, with practical relevance unknown).
May have some anti-diabetic effects indpendent of inhibiting absorption of carbohydrates, but these do not appear to be too notable
A 70% ethanolic extract from the roots of Morus Alba to streptozotocin-induced diabtic rats at 600mg/kg bodyweight (200-400mg/kg not being significant) for 10 days effectively normalized serum glucose, insulin and lipid peroxides while normalizing body weight; suggesting protective effects on the pancreas (target of streptozotocin toxicity). A larger dose in this same rat model (1,000mg/kg) was able to reduce blood glucose by 22% over 6 weeks of oral ingestion paired with a reduction in HbA1c to 75% of the untreated group.
In KK-Ay diabetic mice (spontaneously diabetic and fed a high sucrose diet), a 50% ethanolic extract of the leaves standardized to 0.77% 1-deoxynojirimycin (DNJ) noted a slight reduction in food intake at 3% and 6% of feed intake as mulberry extract but not enough to significantly alter body weight over 60 days; a significant reduction in blood glucose occured in a dose-dependent manner in this study.
In Alloxan-induced diabetic mice, Morus Alba with Silkworm powder (4,000mg/kg, or 0.4% of the diet) was able to reduce serum glucose by 50% relative to untreated control. This research model has experienced a 61% reduction of serum glucose in response to the water extract of Morus Alba leaves with an enhanced content of 1-deoxynojirimycin (5% of extract; link to Korean full text) and alloxan-induced diabetic mice also see reductions in blood glucose in a dose-dependent manner with the isolated compounds Moracin M, Steppogenin-4'-O-beta-D-glucoside (a flavonoid), and Mullberroside A (stilbene glycoside) at the oral dose of 50-100mg/kg each over 3 days, although none of these outperformed 50mg/kg gliclazide.
These effects extend to normal male Wistar rats given Morus Alba, where the ethanolic extract of Morus Alba was shown to reduce carbohydrate absorption with IC50 values of 0.11 g/kg (sucrose), 0.44 g/kg (maltose), and 0.38 g/kg (starch). In otherwise healthy Sprague-Dawley rats given 500-1,000mg/kg of Morus Alba ethanolic extract (0.11% 1-deoxynojirimycin) using 5mg/kg Arcabose as an active control, Morus Alba was comparable to Arcabose in reducing the spikes in blood glucose in response to a sucrose or maltose load but underperformed in a starch load. Fecal analysis confirmed undigested carboydrates in response to sucrose and maltose, but failed to find significant evidence for undigested starches in response to Morus Alba ingestion over 3 weeks, although this was noted as a positive in this study as excessive starch malabsorption from Arcabose resulted in reduced intestinal transporting capacity and this reduction was not noted with Morus Alba; starch malabsorption is thought to underlie the only side-effects with Arcabose, namely abdominal distention, flatulence, meteorism, and possibly diarrhea.
Studies that use normal rats but fed a high fat diet note that 1% Mulberries (0.02% 1-deoxynojirimycin) or an equivalent amount of Quercetin-3,6-Malonylglucoside (0.01%; 0.7mg/kg) for 8 weeks reduce plasma glucose (20.4% Mulberry; 31% Q3MG), free fatty acids (Q3MG only; 44%) and LDL-C (Q3MG only; 70%) relative to high fat control; no influence on body weight was noted in either group, and no influence on food intake in the Q3MG group (Mulberry ate 21% more, but did not appear to gain weight).
A large amount of repeated studies in rats showing reductions in blood glucose in all apparent states (food or toxin-induced diabetes as well as normal healthy rats) where Morus Alba and primarily its 1-deoxynojirimycin content reduce fasting blood glucose (diabetic rats) or post-prandial spikes in glucose from ingested food (diabetic and normal rats)
Some compounds derived from the methanolic (and ethyl acetate) fractions of Morus Alba root barks show NF-kB inhibitory potential, with IC50 values of 4.65uM (Kuwanon J 2,4,10″-trimethyl ether) and 7.38uM (Kuwanon R), both of which were able to inhibit NF-kB by 87% and 75% (respectively) at 5ug/mL; both underperformed relative to the reference compound Celastrol (from Thunder God Vine) with an IC50 of 4.1+/-1.05uM. Sanggenon C and O also appear to inhibit NF-kB, and their respective IC50 values of 3.38 and 1.29uM appear to be comparable to Celastrol, and in this study was noted to be comparable to deguelin.
Arylbenzofuran compounds (usually named 'Moracin' in this plant) and prenylated flavonoids also show anti-inflammatory potential by inhibiting nitric oxide release from macrophages, with the former class having IC50 values ranging from 7.1-19.3uM (n=10) and the latter class ranging from 10.5-19.0uM (n=5). Many compounds in these two classes outperformed aminoguanidine (17.5uM) and the saponin structures were fairly inactive (greater than 100uM). The prenylated flavone known as Cudraflavone B (THP-1 cytotoxicity at 47.6uM) can inhibit TNF-α gene trancription after LPS stimulation to a potency greater than indomethacin at 10uM, inhibited COX-2 induction with an IC50 of 2.5+/-0.89uM (slightly weaker than indomethacin, but with greater selectivity at 1.70-fold more for COX1 than COX2) and inhibited nuclear translocation of NF-kB by 2.3-fold (relative to control).
Sanggenons C, E, and O appear to inhibit COX-1 and COX-2 with potencies ranging from 10-14μM and 40-50μM, respectively, with Moracin-structured compounds being ineffective. which may be downstream of NF-kB inhibition (seen with C and O).
Bioactives have, in vitro, fairly potent anti-inflammatory properties; more potent than many nutraceuticals but they do not appear to be among the most potent (Thunder God Vine and Feverfew appear to still be more effective on these parameters)
In macrophages, two pyrrole compounds (2-formyl-5-(hydroxymethyl)-1H-pyrrole-1-butanoic acid and 2-formyl-5-(methoxymethyl)-1H-pyrrole-1-butanoic acid) were able to activate macrophages in the concentration range of 10-100uM as assessed by nitric oxide release, phagocytic activity, and release of cytokines (IL-12 and TNF-α); the potency was lesser than that of LPS-induced activity and no group altered cell viability.
Some compounds may have immunostimulatory properties, with practical potency of these unknown
In a database search for ADAMTS1 inhibitors, Kuwanon P and X as well as both albafuran C and mulberrofuran J from Morus Alba where able to inhibit the enzyme in bacterial-expressed cultures and human cell cultures with IC50 values of 5.5-7.6uM, 15.5-16.2uM, 10.7-11.9uM, and 18.4-25.4uM respectively. ADAMTS1 is a protein complex that has catalytic activities towards proteoglycans such as aggrecan, versican and brevican which are major components of articular cartilage; its inhibition is throught to be therapeutic for arthritis.
Therapeutic efficacy in arthritis not yet known, but components of White Mulberry appear to be theoretically therapeutic by a novel mechanism
One mouse study using the cortex (outer part of root) at 50-200mg/kg (with 10mg/kg Cyclosporin A as active control) for 6 weeks (5 days a week) noted that only the 200mg/kg group reduced airway hyperresponsiveness (a measurement of allergic asthma) to a similar degree as Cyclosporin A, although 50mg/kg was not at all different than control rats. These observations occurred with reduced airway inflammation and collagen deposition in the lungs, reduced eosinophil count (no influence on basophils or monocytes) and reduced IgE and Histamine secondary to reduced Th2 cell proliferation.
The ethanolic extract of the branch of Morus Alba between 10-40mg/kg in mice with hyperuricemia (high blood uric acid) noted that this extract, with a high mulberroside A content (1.5%), was able to attenuate the increase in uric acid induced by potassium oxonate wholly at 20-40mg/kg and attenuate the increase at 10mg/kg. Mulberroside A is the bioactive causative of these effects.
Mori Alba showed comparable efficacy to the active control of 100mg/kg probenecid, and was associated with normalizing the upregulation of mURAT1 and mGLUT9 mRNA levels and the downregulation of mOAT1 (all of which are perturbed in hyperuricemia and involved in its regulation); along these lines, Morus Alba failed to reduce serum uric acid in normal mice, and preserved renal function in mice with high blood uric acid.
May attenuate increases in uric acid by promoting its urinary excretion
One study using HL-60 cells noted that Albinol A was able to inhibit cell proliferation with an IC50 of 1.7uM, which was comparable to Cisplatin (IC50 1.9uM) with the other tested compound being subpar (Mulberrofurane Q; IC50 of 37.6uM). Albinol A appears to be a topoisomerase type II inhibitor with an IC50 of 22.8uM which was comparable to Etoposide, although the potency of Albinol A on Topoisomerase type I was much lesser (88.4uM) and underperformed relative to Camptothecin; this is thought to underlie apoptosis in this cell line.
A study using CRL1579 melanoma cells stated that Albinol A was comparable to Cisplatin in regards to IC50 value in inhibiting cell proliferation in vitro, although the IC50 for Albinol A (9.8uM) was seemingly less than Cisplatin (21.1uM).
A lectin (known as MLL) isolated from Morus Alba noted an IC50 value of inhibiting cell proliferation of 8.5mcg/mL in MCF-7 cells, underperforming relative to Cisplatin (2mcg/mL); the morphology of cells undergoing growth inhibition is similar to that observed with apoptosis by Cisplatin and associated with Caspase-3 release.
In HCT-15 (colorectal cancer cells), a lectin known as MLL has an IC50 value of inhibiting cell proliferation of 16mcg/mL, underperforming relative to Cisplatin (1mcg/mL). These anti-proliferative effects are mediated via apoptosis related to Caspase-3 release, and are morphologically similar to Cisplatin-induced apoptosis.
Mulberry extracts appears to have similar tyrosinase-inhibiting potential as hydroquinone and kojic acid, two reference compounds. The bioactive responsible may be Mulberroside A (Oxyresveratrol diglucoside), although Mulberroside A is weaker than its metabolite Oxyresveratrol, with both compounds being more effective than arbutin. Oxyresveratrol has been demonstrated to reduce tyrosinase activity to 62.6+/-1.4% of control (Mulberroside A to 78.6%, Arbutin to 82.4%) and reduced melanin concentrations in a dose-dependent manner, and biotransformation of Mulberroside A into its aglycones has been noted elsewhere to enhance anti-tyrosinase activity, where oxyresveratrol demonstrated mixed competitive inhibition of Tyrosinase in response to L-Tyrosine and noncompetitive to L-DOPA. These benefits may extend to Morus Nigra due to sharing bioactives.
In a trail on Melasma in persons with Fitzpatrick phototypes III-IV (dark white to light brown skin tone) advised to apply a 75% Mulberry oil (in coconut oil base) to their problematic skin twice daily for a period of 8 weeks noted significant improvements on symptoms of Melasma as assessed by both the Melasma Area and Severity Index (MASI) and Mexameter readings; quality of life improved with Mulberry oil but not placebo.
The only human study on Morus Alba at this time is one using the oils topically, where the anti-tyrosinase and skin whitening capabilities were demonstrated to be better than placebo
Administration of the water extract of Mori Albae at 858.2+/-29.5mg/kg bodyweight to rats was able to decrease serum levels of ethanol when given either 30 minutes before or simultaneously with ethanol and preserve serum levels of ethanol when given to rats 30 minutes after ethanol (3g/kg bodyweight) ingestion. Mori Albae was able to attenuate the decrease in Alcohol Dehydrogenase activity that occurs with alcohol ingestion (less potent than Dolichorus Semen and Alpiniae Katsumadai), despite other compounds like hovenia dulcis failing to do so.
A compound in Morus Alba known as albosteroid also appears to exert protective effects against ethanol-induced gastric ulcers in a dose-dependent manner.
May increase the metabolism rate of alcohol when ingested before alcohol or preserve the serum level of alcohol when coingested (due to being less effective at reducing serum ethanolic and merely attenuates a further increase). Note that serum levels correlate with the state of drunk, and preloading Morus Alba (if effective in reducing the perception of drunk; no human evidence on this) may require more alcohol to 'get the job done'
One study that used four Traditional Chinese Medicine herbs together noted that the combination of Morus Alba (0.48mg/mL) with Schisandra chinensis (3mg/mL), Coptis Chinensis (80mcg/mL), and Psidium guajava leaves (374.56mcg/mL) showed alpha-glucosidase inhibitory potential.
In a high fat and high sucrose fed group of rats that developed diabetes, 10.25, 20.50, or 51.25mL/kg of this ratio daily for 9 weeks (totalling 4.92-24.6mg/kg Morus Alba, 30.75-153.75mg/kg schisandra chinensis, 820-4100mcg/kg Coptis, and 3.8-19.2mg/kg Psidium) noted that acutely, only the highest dose was able to attenuate the spike in blood glucose seen with an oral glucose tolerance test to the levels seen in normal mice 30 minutes after ingestion; with all groups being equally effective between 60-120 minutes but not significantly more than control. All groups appeared to be simiarly effective in an insulin tolerance test, and fasting blood glucose decreased in a dose-dependent manner over the nine weeks with the high dose group normalizing 62% of the increase in seen in diabetic control versus lean control.
Roselle (Hibiscus sabdariffa) appears to be synergistic with Morus Alba in inhibiting carbohydrate digestive enzymes in vitro, where their individual inhibitory potential on pancreatic α-amylase at 2mg/mL was 18.99+/-1.39% and 1.17+/-0.74% (respectively) and the combination was 65.75+/-0.60% inhibition; this synergism with roselle extends to Chrysanthemum Indicus and Aegle marmelos.
Hibiscus Sabdariffa appears to be synergistic with a few herbs in inhibiting pancreatic amylase activity (starch digestive enzyme), of which Morus Alba is one of them
A study using the bioactive Morin noted that consumption og 10mg/kg Morin for 7 days prior to a Piracetam load of 50mg/kg was able to increase the subsequent Piracetam AUC by approximately 50% and the Cmax of Piracetam by 45%, credited to inhibition of CYP3A4 and P-Gyp. A single dose of Morin was unable to mimic these effects, and this oral dose of Morin can be attained with 813mg/kg of the bark (highest Morin concentration) or more practically with ethanolic extracts, which have been noted to concentration Morin up to 0.5mg/g (and thus would require 20mg/kg).
Possible synergism with a diet rich in Morin-containing foods such as Morus Alba with Piracetam, but the oral dose required may denote supplementation of Morin is needed rather than food sources (which are comparatively low to the oral dose used)
One study assessing the usage of Morus Alba at 100mg/kg in pregnant rats (either healthy, diabetic, hypercholesterolemic, or both diabetic and hypercholesterolemic mother rats) that noted increases in glucose and retinal pathology (increased risk for cataract formation) noted that Morus Alba exerted protective effects on the mother rats, and less adverse events extended to the pups once they were born (although this was said to be due to lessened pathological signs, and not an inherently beneficial effect of Morus Alba on pups).
- Hemmer W, et al. Identification of Bet v 1-related allergens in fig and other Moraceae fruits. Clin Exp Allergy. (2010)
- Kikuchi T, et al. Albanol A from the root bark of Morus alba L. induces apoptotic cell death in HL60 human leukemia cell line. Chem Pharm Bull (Tokyo). (2010)
- Nomura T, Hano Y, Fukai T. Chemistry and biosynthesis of isoprenylated flavonoids from Japanese mulberry tree. Proc Jpn Acad Ser B Phys Biol Sci. (2009)
- Shi YW, et al. Uricosuric and nephroprotective properties of Ramulus Mori ethanol extract in hyperuricemic mice. J Ethnopharmacol. (2012)
- Nam S, Jang HW, Shibamoto T. Antioxidant activities of extracts from teas prepared from medicinal plants, Morus alba L., Camellia sinensis L., and Cudrania tricuspidata , and their volatile components. J Agric Food Chem. (2012)
- Iqbal S, et al. Proximate Composition and Antioxidant Potential of Leaves from Three Varieties of Mulberry (Morus sp.): A Comparative Study. Int J Mol Sci. (2012)
- Mahmood T, et al. Effect of maturity on phenolics (phenolic acids and flavonoids) profile of strawberry cultivars and mulberry species from pakistan. Int J Mol Sci. (2012)
- Influence of alcoholic fermentation process on antioxidant activity and phenolic levels from mulberries (Morus nigra L.).
- Imran M, et al. Chemical composition and antioxidant activity of certain Morus species. J Zhejiang Univ Sci B. (2010)
- Chu Q, et al. Study on capillary electrophoresis-amperometric detection profiles of different parts of Morus alba L. J Chromatogr A. (2006)
- Chen SK, et al. Moracin M from Morus alba L. is a natural phosphodiesterase-4 inhibitor. Bioorg Med Chem Lett. (2012)
- Arias L, Vara Y, Cossío FP. Regioselective preparation of benzo(b)furans from phenols and α-bromoketones. J Org Chem. (2012)
- Yang ZG, et al. Inhibitory effects of constituents from Morus alba var. multicaulis on differentiation of 3T3-L1 cells and nitric oxide production in RAW264.7 cells. Molecules. (2011)
- Yang Y, et al. Four new 2-arylbenzofuran derivatives from leaves of Morus alba L. Chem Pharm Bull (Tokyo). (2010)
- Confirmation of the structures of kuwanons G and H (albanins F and G) by partial synthesis.
- Fukai T, et al. Structures of two natural hypotensive Diels-Alder type adducts, mulberrofurans F and G, from the cultivated mulberry tree (Morus lhou KOIDZ.). Chem Pharm Bull (Tokyo). (1985)
- Geng C, et al. New isoprenylated flavonoid from Morus alba. Zhongguo Zhong Yao Za Zhi. (2010)
- Geng CA, et al. Mulberrofuran G and isomulberrofuran G from Morus alba L.: anti-hepatitis B virus activity and mass spectrometric fragmentation. J Agric Food Chem. (2012)
- Smejkal K, et al. Cytotoxic activities of several geranyl-substituted flavanones. J Nat Prod. (2010)
- Phung TX, et al. Chalcone-derived Diels-Alder adducts as NF-κB inhibitors from Morus alba. J Asian Nat Prod Res. (2012)
- Dat NT, et al. Cytotoxic prenylated flavonoids from Morus alba. Fitoterapia. (2010)
- Kim HJ, et al. HPLC-based activity profiling--discovery of sanggenons as GABAA receptor modulators in the traditional Chinese drug Sang bai pi (Morus alba root bark). Planta Med. (2012)
- Rollinger JM, et al. Discovering COX-inhibiting constituents of Morus root bark: activity-guided versus computer-aided methods. Planta Med. (2005)
- Kim SB, et al. Macrophage activating activity of pyrrole alkaloids from Morus alba fruits. J Ethnopharmacol. (2013)
- Hunyadi A, et al. In vitro Anti-diabetic Activity and Chemical Characterization of an Apolar Fraction of Morus alba Leaf Water Extract. Phytother Res. (2012)
- Ali A, Ali M. New triterpenoids from Morus alba L. stem bark. Nat Prod Res. (2012)
- Yang Y, et al. Two novel flavanes from the leaves of Morus alba L. J Asian Nat Prod Res. (2010)
- Lee JJ, et al. Morusinol extracted from Morus alba inhibits arterial thrombosis and modulates platelet activation for the treatment of cardiovascular disease. J Atheroscler Thromb. (2012)
- Yang Y, et al. Two new chalcones from leaves of Morus alba L. Fitoterapia. (2010)
- Kim YJ, Sohn MJ, Kim WG. Chalcomoracin and moracin C, new inhibitors of Staphylococcus aureus enoyl-acyl carrier protein reductase from Morus alba. Biol Pharm Bull. (2012)
- Ahmad A, et al. Antiulcer and antioxidant activities of a new steroid from Morus alba. Life Sci. (2012)
- Hošek J, et al. Natural compound cudraflavone B shows promising anti-inflammatory properties in vitro. J Nat Prod. (2011)
- Oh H, et al. Hepatoprotective and free radical scavenging activities of prenylflavonoids, coumarin, and stilbene from Morus alba. Planta Med. (2002)
- Weber JT, et al. Potential neuroprotective effects of oxyresveratrol against traumatic injury. Eur J Pharmacol. (2012)
- Chemical constituents from the water extracts of Cortex Mori.
- Piao SJ, et al. Simultaneous determination of five characteristic stilbene glycosides in root bark of Morus albus L. (Cortex Mori) using high-performance liquid chromatography. Phytochem Anal. (2011)
- Zhang M, et al. In vivo hypoglycemic effects of phenolics from the root bark of Morus alba. Fitoterapia. (2009)
- Yang Y, Wang HQ, Chen RY. Flavonoids from the leaves of Morus alba L. Yao Xue Xue Bao. (2010)
- Oh BK, et al. Melanin-concentrating hormone-1 receptor binding activity of pheophorbides isolated from Morus alba leaves. Phytother Res. (2010)
- Dugo P, et al. Characterization of the polyphenolic fraction of Morus alba leaves extracts by HPLC coupled to a hybrid IT-TOF MS system. J Sep Sci. (2009)
- Hunyadi A, et al. Chlorogenic Acid and Rutin Play a Major Role in the In Vivo Anti-Diabetic Activity of Morus alba Leaf Extract on Type II Diabetic Rats. PLoS One. (2012)
- Nuengchamnong N, et al. Quantitative determination of 1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. (2007)
- Amézqueta S, et al. Determination of D-fagomine in buckwheat and mulberry by cation exchange HPLC/ESI-Q-MS. Anal Bioanal Chem. (2012)
- Asano N, et al. Polyhydroxylated alkaloids isolated from mulberry trees (Morusalba L.) and silkworms (Bombyx mori L.). J Agric Food Chem. (2001)
- A derivatization procedure for the simultaneous analysis of iminosugars and other low molecular weight carbohydrates by GC–MS in mulberry (Morus sp.).
- Kozlov VV, Danilov LL. Separation of polyprenyl phosphate oligomerhomologues by reversed-phase ion-pair high-performance liquid chromatography. Anal Sci. (2012)
- Separation and character analysis of anthocyanins from mulberry (Morus alba L.) pomace.
- Tao Y, et al. Rapid screening and identification of α-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC/MS and NMR. Biomed Chromatogr. (2012)
- Zhang W, et al. HPLC-DAD-ESI-MS/MS analysis and antioxidant activities of nonanthocyanin phenolics in mulberry (Morus alba L.). J Food Sci. (2008)
- Chang LW, et al. Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark. Food Chem Toxicol. (2011)
- Deepa M, et al. Purified mulberry leaf lectin (MLL) induces apoptosis and cell cycle arrest in human breast cancer and colon cancer cells. Chem Biol Interact. (2012)
- Elemental distribution in summer fruits of Pakistan.
- Duran A, Tuzen M, Soylak M. Trace element levels in some dried fruit samples from Turkey. Int J Food Sci Nutr. (2008)
- Altundag H, Tuzen M. Comparison of dry, wet and microwave digestion methods for the multi element determination in some dried fruit samples by ICP-OES. Food Chem Toxicol. (2011)
- Kim ES, et al. Purification and characterization of Moran 20K from Morus alba. Arch Pharm Res. (1999)
- Hikino H, et al. Isolation and hypoglycemic activity of moran A, a glycoprotein of Morus alba root barks. Planta Med. (1985)
- Adisakwattana S, et al. In vitro inhibitory effects of plant-based foods and their combinations on intestinal α-glucosidase and pancreatic α-amylase. BMC Complement Altern Med. (2012)
- Wang W, et al. In vitro antioxidant and antimicrobial activity of extracts from Morus alba L. leaves, stems and fruits. Am J Chin Med. (2012)
- Arfan M, et al. Antioxidant activity of mulberry fruit extracts. Int J Mol Sci. (2012)
- Jaruchotikamol A, Pannangpetch P. Cytoprotective activity of mulberry leaf extract against oxidative stress-induced cellular injury in rats. Pak J Pharm Sci. (2013)
- Kim GN, Jang HD. Flavonol content in the water extract of the mulberry (Morus alba L.) leaf and their antioxidant capacities. J Food Sci. (2011)
- Qiu F, et al. Pharmacological properties of traditional medicines. XXII. Pharmacokinetic study of mulberroside A and its metabolites in rat. Biol Pharm Bull. (1996)
- Mei M, et al. In vitro pharmacokinetic characterization of mulberroside A, the main polyhydroxylated stilbene in mulberry (Morus alba L.), and its bacterial metabolite oxyresveratrol in traditional oral use. J Agric Food Chem. (2012)
- Zhaxi M, et al. Three major metabolites of mulberroside A in rat intestinal contents and feces. Planta Med. (2010)
- Yu MC, et al. Luteolin, a non-selective competitive inhibitor of phosphodiesterases 1-5, displaced (3H)-rolipram from high-affinity rolipram binding sites and reversed xylazine/ketamine-induced anesthesia. Eur J Pharmacol. (2010)
- Ko WC, et al. Inhibitory effects of flavonoids on phosphodiesterase isozymes from guinea pig and their structure-activity relationships. Biochem Pharmacol. (2004)
- Park SJ, et al. Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. Cell. (2012)
- van Staveren WC, et al. The effects of phosphodiesterase inhibition on cyclic GMP and cyclic AMP accumulation in the hippocampus of the rat. Brain Res. (2001)
- Rolipram, a type IV-specific phosphodiesterase inhibitor, facilitates the establishment of long-lasting long-term potentiation and improves memory.
- Wiescholleck V, Manahan-Vaughan D. PDE4 inhibition enhances hippocampal synaptic plasticity in vivo and rescues MK801-induced impairment of long-term potentiation and object recognition memory in an animal model of psychosis. Transl Psychiatry. (2012)
- Yang X, et al. HPLC-based activity profiling for GABAA receptor modulators from the traditional Chinese herbal drug Kushen (Sophora flavescens root). Mol Divers. (2011)
- Hui KM, et al. Anxiolytic effect of wogonin, a benzodiazepine receptor ligand isolated from Scutellaria baicalensis Georgi. Biochem Pharmacol. (2002)
- Yadav AV, Nade VS. Anti-dopaminergic effect of the methanolic extract of Morus alba L. leaves. Indian J Pharmacol. (2008)
- Kim HG, Oh MS. Memory-enhancing effect of Mori Fructus via induction of nerve growth factor. Br J Nutr. (2012)
- Nade VS, et al. Adaptogenic effect of Morus alba on chronic footshock-induced stress in rats. Indian J Pharmacol. (2009)
- Bhuiyan MI, et al. The Neuroprotective Potential of Cyanidin-3-glucoside Fraction Extracted from Mulberry Following Oxygen-glucose Deprivation. Korean J Physiol Pharmacol. (2011)
- Nade VS, Kawale LA, Yadav AV. Protective effect of Morus alba leaves on haloperidol-induced orofacial dyskinesia and oxidative stress. Pharm Biol. (2010)
- Nade VS, Yadav AV. Anti-stress effect of ethyl acetate soluble fraction of Morus alba in chronic restraint stress. Pharm Biol. (2010)
- Yadav AV, Kawale LA, Nade VS. Effect of Morus alba L. (mulberry) leaves on anxiety in mice. Indian J Pharmacol. (2008)
- Sun X, et al. Screening of pancreatic lipase and alpha-glucosidase inhibitors from Chinese dietary herbs. Zhongguo Zhong Yao Za Zhi. (2012)
- Hassan AM. TLC bioautographic method for detecting lipase inhibitors. Phytochem Anal. (2012)
- Duangjai A, Ingkaninan K, Limpeanchob N. Potential mechanisms of hypocholesterolaemic effect of Thai spices/dietary extracts. Nat Prod Res. (2011)
- Chemistry and biological activities of isoprenylated flavonoids from medicinal plants (moraceous plants and Glycyrrhiza species).
- STRUCTURES OF MORACENINS, HYPOTENSIVE PRINCIPLES OF MORUS ROOT BARK.
- Structure of moracenin B, a hypotensive principle of Morus root barks.
- Lee YJ, et al. Hypotensive, hypolipidemic, and vascular protective effects of Morus alba L. in rats fed an atherogenic diet. Am J Chin Med. (2011)
- Enkhmaa B, et al. Mulberry (Morus alba L.) leaves and their major flavonol quercetin 3-(6-malonylglucoside) attenuate atherosclerotic lesion development in LDL receptor-deficient mice. J Nutr. (2005)
- Pirvulescu MM, et al. Curcumin and a Morus alba extract reduce pro-inflammatory effects of resistin in human endothelial cells. Phytother Res. (2011)
- Manduteanu I, et al. Similar effects of resistin and high glucose on P-selectin and fractalkine expression and monocyte adhesion in human endothelial cells. Biochem Biophys Res Commun. (2010)
- Ma X, et al. Morus alba leaf extract stimulates 5'-AMP-activated protein kinase in isolated rat skeletal muscle. J Ethnopharmacol. (2009)
- Yang X, Yang L, Zheng H. Hypolipidemic and antioxidant effects of mulberry (Morus alba L.) fruit in hyperlipidaemia rats. Food Chem Toxicol. (2010)
- Hong Y, Kim MY, Yoon M. The anti-angiogenic herbal extracts Ob-X from Morus alba, Melissa officinalis, and Artemisia capillaris suppresses adipogenesis in 3T3-L1 adipocytes. Pharm Biol. (2011)
- Kobayashi Y, et al. Ameliorative effects of mulberry (Morus alba L.) leaves on hyperlipidemia in rats fed a high-fat diet: induction of fatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stress. Biosci Biotechnol Biochem. (2010)
- Naowaboot J, et al. Mulberry leaf extract stimulates glucose uptake and GLUT4 translocation in rat adipocytes. Am J Chin Med. (2012)
- Saito Y, et al. Molecular characterization of the melanin-concentrating-hormone receptor. Nature. (1999)
- Luthin DR. Anti-obesity effects of small molecule melanin-concentrating hormone receptor 1 (MCHR1) antagonists. Life Sci. (2007)
- Oh KS, et al. Melanin-concentrating hormone-1 receptor antagonism and anti-obesity effects of ethanolic extract from Morus alba leaves in diet-induced obese mice. J Ethnopharmacol. (2009)
- Tanabe K, et al. Repeated ingestion of the leaf extract from Morus alba reduces insulin resistance in KK-Ay mice. Nutr Res. (2011)
- Screening of Anti-angiogenic Activity from Plant Extracts.
- Yoon M, Kim MY. The anti-angiogenic herbal composition Ob-X from Morus alba, Melissa officinalis, and Artemisia capillaris regulates obesity in genetically obese ob/ob mice. Pharm Biol. (2011)
- Benalla W, Bellahcen S, Bnouham M. Antidiabetic medicinal plants as a source of alpha glucosidase inhibitors. Curr Diabetes Rev. (2010)
- Kim GN, Kwon YI, Jang HD. Mulberry leaf extract reduces postprandial hyperglycemia with few side effects by inhibiting α-glucosidase in normal rats. J Med Food. (2011)
- P S, et al. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complement Altern Med. (2011)
- Hansawasdi C, Kawabata J. Alpha-glucosidase inhibitory effect of mulberry (Morus alba) leaves on Caco-2. Fitoterapia. (2006)
- Oku T, et al. Similarity of hydrolyzing activity of human and rat small intestinal disaccharidases. Clin Exp Gastroenterol. (2011)
- Oku T, et al. Inhibitory effects of extractives from leaves of Morus alba on human and rat small intestinal disaccharidase activity. Br J Nutr. (2006)
- Gómez L, et al. D-Fagomine lowers postprandial blood glucose and modulates bacterial adhesion. Br J Nutr. (2012)
- Shang Q, Xiang JF, Tang YL. Screening α-glucosidase inhibitors from mulberry extracts via DOSY and relaxation-edited NNR. Talanta. (2012)
- Katsube T, et al. Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in diet-induced obese mice. J Sci Food Agric. (2010)
- Singab AN, et al. Hypoglycemic effect of Egyptian Morus alba root bark extract: effect on diabetes and lipid peroxidation of streptozotocin-induced diabetic rats. J Ethnopharmacol. (2005)
- Naowaboot J, et al. Antihyperglycemic, antioxidant and antiglycation activities of mulberry leaf extract in streptozotocin-induced chronic diabetic rats. Plant Foods Hum Nutr. (2009)
- Hypoglycemic Effects of Pills Made of Mulberry Leaves and Silkworm Powder in Streptozotocin-Induced Diabetic Rats.
- Hypoglycemic effect of mulberry leaves with anaerobic treatment in alloxan-induced diabetic mice.
- Miyahara C, et al. Inhibitory effects of mulberry leaf extract on postprandial hyperglycemia in normal rats. J Nutr Sci Vitaminol (Tokyo). (2004)
- Chiasson JL, et al. Acarbose for prevention of type 2 diabetes mellitus: the STOP-NIDDM randomised trial. Lancet. (2002)
- Chao WW, et al. The production of nitric oxide and prostaglandin E2 in peritoneal macrophages is inhibited by Andrographis paniculata, Angelica sinensis and Morus alba ethyl acetate fractions. J Ethnopharmacol. (2009)
- Dat NT, et al. Sanggenon C and O inhibit NO production, iNOS expression and NF-κB activation in LPS-induced RAW264.7 cells. Immunopharmacol Immunotoxicol. (2012)
- Peng J, et al. Fluorescence resonance energy transfer assay for high-throughput screening of ADAMTS1 inhibitors. Molecules. (2011)
- Liu YJ, Xu Y, Yu Q. Full-length ADAMTS-1 and the ADAMTS-1 fragments display pro- and antimetastatic activity, respectively. Oncogene. (2006)
- Rodríguez-Manzaneque JC, et al. ADAMTS1 cleaves aggrecan at multiple sites and is differentially inhibited by metalloproteinase inhibitors. Biochem Biophys Res Commun. (2002)
- Kuno K, et al. ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan. FEBS Lett. (2000)
- O'Byrne PM, Inman MD. Airway hyperresponsiveness. Chest. (2003)
- Kim HJ, et al. Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4(+)CD25(+)Foxp3(+) regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model. J Ethnopharmacol. (2011)
- Wang CP, et al. Mulberroside a possesses potent uricosuric and nephroprotective effects in hyperuricemic mice. Planta Med. (2011)
- Glut9 is a major regulator of urate homeostasis and its genetic inactivation induces hyperuricosuria and urate nephropathy.
- Vitart V, et al. SLC2A9 is a newly identified urate transporter influencing serum urate concentration, urate excretion and gout. Nat Genet. (2008)
- Deepa M, Priya S. Purification and characterization of a novel anti-proliferative lectin from Morus alba L. leaves. Protein Pept Lett. (2012)
- Badreshia-Bansal S, Draelos ZD. Insight into skin lightening cosmeceuticals for women of color. J Drugs Dermatol. (2007)
- Park KT, et al. Inhibitory effect of mulberroside A and its derivatives on melanogenesis induced by ultraviolet B irradiation. Food Chem Toxicol. (2011)
- Kim JK, et al. Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition. J Ind Microbiol Biotechnol. (2010)
- Zheng ZP, et al. Tyrosinase inhibitory constituents from the roots of Morus nigra: a structure-activity relationship study. J Agric Food Chem. (2010)
- Alvin G, et al. A comparative study of the safety and efficacy of 75% mulberry (Morus alba) extract oil versus placebo as a topical treatment for melasma: a randomized, single-blind, placebo-controlled trial. J Drugs Dermatol. (2011)
- Effect of Water Extracts of Crude Drugs in Decreasing Blood Ethanol Concentrations in Rats.
- Wang HJ, Chiang BH. Anti-diabetic effect of a traditional Chinese medicine formula. Food Funct. (2012)
- Sahu K, Shaharyar M, Siddiqui AA. Effect of Morin on pharmacokinetics of Piracetam in rats, in vitro enzyme kinetics and metabolic stability assay using rapid UPLC method. Drug Test Anal. (2012)
- Kim HG, et al. Evaluation of Samjunghwan, a traditional medicine, for neuroprotection against damage by amyloid-beta in rat cortical neurons. J Ethnopharmacol. (2010)
- El-Sayyad HI, et al. Protective effects of Morus alba leaves extract on ocular functions of pups from diabetic and hypercholesterolemic mother rats. Int J Biol Sci. (2011)